系统生物学中的实验方法

570 次查看
西奈山伊坎医学院
Coursera
  • 完成时间大约为 20 个小时
  • 混合难度
  • 英语
注:本课程由Coursera和Linkshare共同提供,因开课平台的各种因素变化,以上开课日期仅供参考

课程概况

Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging.

A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content.

课程大纲

Introduction

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Deep mRNA Sequencing

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Mass Spectrometry-Based Proteomics

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Midterm Exam

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Flow and Mass Cytometry for Single Cell Protein Levels and Cell Fate

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Live-cell Imaging for Single Cell Protein Dynamics

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Integrating and Interpreting Datasets with Network Models and Dynamical Models

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Final Exam

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